©  The MarlovitsLab 2011-2013


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The MarlovitsLab

@ Kulcar/Marlovits

CryoEM of

Molecular Machines in Action


Design and Structural Plasticity

Function and Disease

stay tuned ....

—most recent publications

Textbook: Molecular Biology of the Cell (Alberts et al)

.... T3SS: During his PhD, Oliver, the first graduate student in our lab, took images of isolated Type III secretion systems.  These are now printed in chapter 23 (“Pathogens and Infection) in the famous textbook Molecular Biology of the Cell (Alberts et al).


.... Mechansims of F-actin bundlers (alpha-actinins): The actin cytoskeletal is a dynamic network of actin filaments and F-actin binding proteins such as alpha-actinins.  In a collaborative work with the Djinovic-Carugo lab we show how Calcium modulated domain flexibility of alpha-actinins thus regulate actin bundling.  Luciano was leading the electron microscopy part of that integrative structural study. Great work!

In collaboration with the Djinovic-Carugo (U Vienna/MFPL) lab.

doi: 10.1073/pnas.1917269117

Communications Biology

.... T3SS: Bacteria Exploited for Therapeutic Applications. Will we be harnessing the protein translocation power of the T3SS to treat various diseases in the future?  This work demonstrates how such a system can be designed. In a collaborative effort, Antoine (graduate student in our lab and now post-doc in the Penninger lab) developed the basic principles for reprogramming the secretion system to deliver therapeutic molecules directly into cells. Fantastic!

In collaboration with the Penninger-Lab (IMBA/Vienna, LSI/Vancouver), Plückthun-Lab (U. Zürich), and Rabbits-Lab (Oxford)


Curr Top Microb Immunol.

.... T3SS: Book chapter in Current Topics in Microbiology and Immunology wtihin the series “Bacterial Type III Secretion Systems”: The structure of the Type III Secretion System Needle Complex - by Sean and Niki

doi: 10.1007/82_2019_178


.... T3SS: Elucidation of the complete assembly pathway of the T3SS needle complex at 2.5 Angstroem of resolution - “Structural control for the coordinated assembly into functional pathogenic type-3 secretion systems”. Fantastic work from Nikolaus, Vadim, and Matthias, including many others in the lab, who contributed to fully understand the assembly process for the first time at unprecedented high resolution|

In collaboration with the DiMaio (Seattle), Wagner (Tübingen), Lea (Oxford) Labs


.... Human rhinovirus: Identification of an unexpected binding site for the antiviral drug OBR-5-340 determined by cryoEM. Great work from Jirka in the lab, who uncovered the site! In collaboration with Kirchmair (Bergen), Blaas (Vienna), Schmidtke (Jena) Labs

doi: 10.1073/pnas.1904732116


.... Technology: High-throughput screening for detergent-solubilized membrane proteins. Congratulations to Vadim in the lab!

In collaboration with Garcia-Ali (Hamburg)

doi: 10.1038/s41598-019-46686-8


.... T3SS: The type-3 secretion system needle filament is structurally polymorph.

In collaboration with the J. Galan (Yale) and A Loquet (Bordeaux) labs.

doi: 10.1371/journal.pbio.3000351

Nature Microbiology

.... Tuberculosis: Structure of the type VII secretion system from Mycobacteria elucidated for the first time.  The high flexibility of specific domains suggests an as yet unseen mode of substrate recognition. Congratulations to Luciano in the lab, who solved the structure of this highly challenging sample.

In collaboration with the Bittner/Houben (VU Amsterdam) and Wilmanns (EMBL Hamburg) labs.

doi: 10.1038/nmicrobiol.2017.47

Nature communications

....  T3SS: Complete dissection of the regulatory elements within one of the most complex systems known (T3SS) to date,  and application of basic conceptual principles and tools from synthetic biology to refactor and build a complete functional T3SS.

In collaboration with the Voigt lab (MIT/Boston).

doi: 10.1038/ncomms14737

PLoS Pathogens

.... T3SS: Biochemical, native mass spec and EM-characterization of the export apparatus of the T3SS.

In collaboration with the Robinson (Oxford), Galan (Yale) and Wagner (Tübingen) labs.

doi: 10.1371/journal.ppat.1006071

Nature methods

.... Technology: Novel methods paper for accurate atomic structure determination from density maps.

In collaboration with the Baker Lab (Seattle)

doi 10.1038/nmeth.3286

Annual Review in Microbiology

.... review “Bacterial type III secretion systems: specialized nanomachines for protein delivery into target cells”

doi: 10.1146/annurev-micro-092412-155725

Journal of Cell Biology

.... kinetochore paper in JCB.

In collaboration with the Westermann lab (Vienna)

Nature Structural and Molecular Biology

.... T3SS: type III secretion systems in action - in vitro and in situ - first demonstration and visualization of unfolded protein transport across membranes. Congratulations to Julia and Lisa in the lab for their heroic efforts!

doi: 10.1038/nsmb.2722


....  accepting applications for several experts in:

       [molecular biology and/or protein biochemistry] or [electron microscopy] (PostDoc, PhD, M.sc, TA-level)